Genomic and transcriptional analysis of protein heterogeneity of the honeybee venom allergen Api m 6
Wiley -- Insect Molecular Biology
DOI 10.1111/j.1365-2583.2006.00669.x
  1. allelic variation
  2. allergen
  3. Api m 6
  4. bee venom
  5. signal peptide

Several components of honeybee venom are known to cause allergenic responses in humans and other vertebrates. One such component, the minor allergen Api m 6, has been known to show amino acid variation but the genetic mechanism for this variation is unknown. Here we show that Api m 6 is derived from a single locus, and that substantial protein-level variation has a simple genome-level cause, without the need to invoke multiple loci or alternatively spliced exons. Api m 6 sits near a misassembled section of the honeybee genome sequence, and we propose that a substantial number of indels at and near Api m 6 might be the root cause of this misassembly. We suggest that genes such as Api m 6 with coding-region or untranslated region indels might have had a strong effect on the assembly of this draft of the honeybee genome.