Sci Rep
Nature Publishing Group UK
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Three-dimensional architecture of human diabetic peripheral nerves revealed by X-ray phase contrast holographic nanotomography
Volume: 10
DOI 10.1038/s41598-020-64430-5

Highlights

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Abstract

A deeper knowledge of the architecture of the peripheral nerve with three-dimensional (3D) imaging of the nerve tissue at the sub-cellular scale may contribute to unravel the pathophysiology of neuropathy. Here we demonstrate the feasibility of X-ray phase contrast holographic nanotomography to enable 3D imaging of nerves at high resolution, while covering a relatively large tissue volume. We show various subcomponents of human peripheral nerves in biopsies from patients with type 1 and 2 diabetes and in a healthy subject. Together with well-organized, parallel myelinated nerve fibres we show regenerative clusters with twisted nerve fibres, a sprouted axon from a node of Ranvier and other specific details. A novel 3D construction (with movie created) of a node of Ranvier with end segment of a degenerated axon and sprout of a regenerated one is captured. Many of these architectural elements are not described in the literature. Thus, X-ray phase contrast holographic nanotomography enables identifying specific morphological structures in 3D in peripheral nerve biopsies from a healthy subject and from patients with type 1 and 2 diabetes.

Keywords
https://www.researchpad.co/tools/openurl?pubtype=article&doi=10.1038/s41598-020-64430-5&title=Three-dimensional architecture of human diabetic peripheral nerves revealed by X-ray phase contrast holographic nanotomography&author=&keyword=Peripheral neuropathies,Diabetes,Other nanotechnology,&subject=Article,