Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
International Scientific Literature, Inc.
Differential Metabolic Pathways and Metabolites in a C57BL/6J Mouse Model of Alcoholic Liver Disease
Volume: 26
DOI 10.12659/MSM.924602

BackgroundAlcoholic liver disease (ALD), an important cause of acute or chronic liver injury, results from binge drinking or long-term alcohol consumption. To date, there is no well-established mouse model with a comprehensive metabolic profile that mimics ALD in humans. This study aimed to explore the differential metabolic pathways and related differential metabolites in the liver of an ALD mouse model.Material/MethodsA C57BL/6J mouse model of ALD was induced by alcohol feeding for 10 days plus binge alcohol feeding. The metabolomic profiles in the liver of the ALD mouse model was detected through ultra-high-pressure liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC/Q-TOF-MS).ResultsA total 35 metabolites were significantly altered during the development of ALD. These metabolites were correlated to multiple metabolic pathways, including purine metabolism, the pentose phosphate pathway, cysteine and methionine metabolism, D-glutamine and D-glutamate metabolism, pyrimidine metabolism, and vitamin B6 metabolism.ConclusionsThe findings of the present study reveal potential biomarkers of ALD, and provide further insights into the pathogenesis of ALD.

Keywords Metabolic Pathways and Metabolites in a C57BL/6J Mouse Model of Alcoholic Liver Disease&author=Tai Ma,Yue Li,Yun Zhu,Shuling Jiang,Chen Cheng,Zhiwei Peng,Long Xu,&keyword=Lipid Metabolism Disorders,Liver Diseases, Alcoholic,Metabolomics,&subject=Animal Study,