PLoS Genetics
Public Library of Science
image
ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range
Volume: 16, Issue: 4
DOI 10.1371/journal.pgen.1008750
  •       
Abstract

Horizontal gene transfer is the main mechanism by which bacteria acquire and disseminate new traits, such as antibiotic resistance genes, that allow adaptation and evolution. Here we identified a gene, ardC, that enables a plasmid to increase its conjugative host range, and thus positively contributes to plasmid fitness. The crystal structure of the antirestriction protein ArdC revealed a fold different from other antirestriction proteins. Our results have wide implications for understanding how a gene enlarges the environments a plasmid can colonize and point to new targets to harness the bacterial DNA uptake control.

https://www.researchpad.co/tools/openurl?pubtype=article&doi=10.1371/journal.pgen.1008750&title=ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient <i>hsdRMS</i> restriction system broadening conjugation host range&author=Lorena González-Montes,Irene del Campo,M. Pilar Garcillán-Barcia,Fernando de la Cruz,Gabriel Moncalián,Diarmaid Hughes,&keyword=&subject=Research Article,Biology and Life Sciences,Organisms,Bacteria,Pseudomonas,Pseudomonas Putida,Biology and life sciences,Genetics,DNA,Forms of DNA,Plasmids,Biology and life sciences,Biochemistry,Nucleic acids,DNA,Forms of DNA,Plasmids,Biology and Life Sciences,Genetics,Genetic Elements,Mobile Genetic Elements,Plasmids,Biology and Life Sciences,Genetics,Genomics,Mobile Genetic Elements,Plasmids,Biology and Life Sciences,Biochemistry,Enzymology,Enzymes,Proteases,Metalloproteases,Biology and Life Sciences,Biochemistry,Proteins,Enzymes,Proteases,Metalloproteases,Biology and life sciences,Molecular biology,Molecular biology techniques,DNA construction,Plasmid Construction,Research and analysis methods,Molecular biology techniques,DNA construction,Plasmid Construction,Biology and Life Sciences,Evolutionary Biology,Evolutionary Processes,Horizontal Gene Transfer,Biology and Life Sciences,Genetics,Gene Transfer,Horizontal Gene Transfer,Biology and life sciences,Biochemistry,Proteins,DNA-binding proteins,Physical Sciences,Physics,Condensed Matter Physics,Solid State Physics,Crystallography,Crystal Structure,Biology and Life Sciences,Molecular Biology,Molecular Biology Techniques,Gene Mapping,Restriction Fragment Mapping,Electrophoretic Mobility Shift Assay,Research and Analysis Methods,Molecular Biology Techniques,Gene Mapping,Restriction Fragment Mapping,Electrophoretic Mobility Shift Assay,