Springer International Publishing
Ongoing Developments and Clinical Progress in Drug-Loaded Red Blood Cell Technologies
Volume: 34, Issue: 3
DOI 10.1007/s40259-020-00415-0

Engineered red blood cells (RBCs) appear to be a promising method for therapeutic drug and protein delivery. With a number of agents in clinical trials (e.g., dexamethasone 21-phosphate in ataxia telangiectasia, asparaginase in pancreatic cancer/acute lymphoblastic leukemia, thymidine phosphorylase in mitochondrial neurogastrointestinal encephalomyopathy, RTX-134 in phenylketonuria, etc.), this leading article summarizes the ongoing efforts in developing these agents, focuses on the clinical progress, and provides a brief background into engineered RBCs and the different ways in which they can be exploited for therapeutic/diagnostic purposes. References to available data on safety, efficacy, and tolerability are reported. Due to the continuous progress in this field, the information is updated as of January 2020 from databases, websites, and press releases of the involved companies and information that is in the public domain.

Rossi, Pierigè, Aliano, and Magnani: Ongoing Developments and Clinical Progress in Drug-Loaded Red Blood Cell Technologies

Key Points

Engineered RBCs have now reached clinical development with some products already in phase III.
Leading companies in phase III include EryDel SpA and Erytech Pharma; others have received FDA and/or EMA clearance for patient recruitment (Rubius Therapeutics and St George’s, University of London with Orphan Technologies).
Orphan drug designation has been obtained by the FDA and EMA for several drug products; several regulatory paths to approval have been defined with the main regulatory agencies.

Engineered Erythrocytes

Red blood cells (RBCs) are the most numerous cells present in the blood. In a human being, 4–5 million RBCs are present in each microliter of blood. By a simple estimate of 5 L of blood in a 70 kg adult, a total of approximately 20–25 × 1012 RBCs can be calculated. In transfusion medicine, it is common practice to collect blood from healthy donors to be transfused into compatible patients in need. This operation, which usually requires 400–450 mL of blood, can be safely repeated many times every year, as our hematopoietic system has the ability to replace the removed RBCs with new erythrocytes with an output of 2.5 million cells per second. Of interest, when mature human RBCs enter into circulation, they can survive for more than 110–120 days and are removed from circulation only when they are senescent. Thus, the removal of the RBCs from the blood is a very sophisticated process capable of distinguishing, among all circulating RBCs, only those that have reached their programmed life span. Human RBCs possess additional advantageous characteristics. Due to an extreme differentiation process, they have lost the nucleus and other organelles and are thus considered a nonviable container of about 90 fl with a biconcave shape and a diameter of 7–8 µm. This apparently simple cell is able to squeeze through human capillaries (which are < 2 µm in diameter) without being damaged and to travel throughout the circulatory system in about 20 s. Because of these special properties, years ago several investigators hypothesized that erythrocytes could be used not only to replace blood lost from trauma or for other reasons, but could represent an invaluable cell source easily available for the delivery of therapeutic and contrasting agents in the circulatory system.

Human erythrocytes have been suggested as carriers and bioreactors since 1973 [1]. The basis for these potential applications derived from the seminal work of Hoffman [2, 3], who demonstrated that pores opened on the erythrocyte membrane in hypotonic conditions can be resealed, restoring the main properties of the native cell membrane. Since then, many groups have documented the feasibility of this approach in different animal species [4] for the encapsulation of therapeutic proteins, antigens, contrasting agents, small chemical entities [5, 6] as well as for the delivery of agents coupled to the external surface of the RBC membrane [79]. Thus, in addition to osmotic-based procedures, RBC engineering can be obtained by multiple strategies, as reviewed in [10, 11]. It is worth noting that RBCs can serve both as a delivery system for the release of drugs in circulation and to keep the therapeutic agent inside the cell while the metabolite to be degraded crosses the RBC membrane. Both approaches are already in clinical studies today.

Clinical Progress

The clinical developments reported in this section have received approval by international regulatory agencies; many have also received the designation of ‘orphan drug’, and the most advanced are based on solid preclinical and/or phase I/II clinical investigations. Table 1 summarizes the therapeutic goals and the drugs discussed in this paper.

Table 1
Therapeutic goals and drugs considered in this article related to the use of drug-loaded red blood cells as therapeutic agents
Condition treatedDrugCompany
Ataxia telangiectasiaDexamethasone 21-phosphate


Italy & USA

Acute lymphoblastic leukemia/pancreatic cancerAsparaginase

ERYtech Pharma

France & USA

Mitochondrial neurogastrointestinal encephalomyopathyThymidine phosphorylase

St George’s, University of London


The Clinical Trial Company


Orphan Technologies Ltd



Rubius USA

Red Blood Cells (RBCs) for the Delivery of Dexamethasone

Dexamethasone is a glucocorticoid analog without mineralocorticoid activity and with the highest potency with respect to hydrocortisone. Because of its chemistry, it can easily cross cell membranes (including the RBC membrane) by simple diffusion. As a consequence, it is not retained within the RBCs upon encapsulation. This problem was solved by Magnani et al. [12] by encapsulating the corticosteroid derivative dexamethasone 21-phosphate (Dexa 21-P). Specific equipment to perform the encapsulation procedure was also developed [13]. The idea of using a non-diffusible prodrug of dexamethasone using autologous erythrocytes was also very beneficial in terms of pharmacokinetics. In fact, Dexa 21-P is slowly converted to the diffusible dexamethasone by RBC resident enzymes and detected in circulation for > 1 month in human volunteers receiving a single infusion of Dexa 21-P-loaded RBCs [14]. In the same study sponsored by EryDel SpA, it was also shown that the processed RBCs have normal in vivo survival and are fully compliant with the rules for FDA-defined transfusion products. Mambrini et al. [13] were also able to document that the human RBCs can be efficiently loaded with a large range of Dexa 21-P concentrations while maintaining the RBCs’ fundamental properties. Dexa 21-P-loaded RBCs have been used in several investigator-initiated clinical studies in chronic obstructive pulmonary disorder (COPD) patients [15], cystic fibrosis [16], steroid-dependent irritable bowel disease (IBD) [17], Crohn’s disease [18, 19], and ulcerative colitis [20, 21], documenting safety and clinical effect in hundreds of patients. Most recently, EryDel SpA started a registration program in ataxia telangiectasia patients. The treatment received orphan drug designation by both the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA). The phase II trial enrolled 22 patients receiving a monthly administration of autologous RBCs loaded ex vivo with Dexa 21-P for 6 months. It was very successful, reaching both primary and secondary endpoints [22], namely improvement of the neurological conditions measured by a validated International Cooperative Ataxia Rating Scale (ICARS) and adaptive behavior measured by the Vineland Adaptive Behavior Scale (VABS), and an acceptable safety profile. Based on these results, EryDel started a phase III registration study. This is an international, multi-center, 1-year, randomized, prospective, double-blind, placebo-controlled phase III study designed to assess the effect of two non-overlapping dose ranges of Dexa 21-P, administered by IV infusion once per month, on neurological symptoms of patients with ataxia telangiectasia. This study is being conducted in 22 centers located in the US, Europe, Israel, Asia, Africa, and Australia (for more details, visit A total of 180 patients will be enrolled equally into three groups of two Dexa 21-P doses and a placebo. Patients completing this study will be allowed to continue treatment in an open-label extension study.

RBCs for the Delivery of Asparaginase

The idea of using RBCs for the delivery of asparaginase was proposed by Ihler et al. [1], but without any experimental evidence for its feasibility. Preclinical investigations in baboons were reported in 1976 [23], and in monkeys in 1983 [24]. In the years 1990–1996, Ropars and colleagues [2529] proved the feasibility and provided evidence that asparaginase-loaded homologous RBCs are safe, well tolerated, and effective in reducing plasma asparagine in humans. Results from a phase I/II clinical trial of asparaginase-loaded RBCs (named GRASPA® , property of Erytech Pharma) in the treatment of refractory or relapsed lymphoblastic leukemia were published in 2011 [30]. In 2015, GRASPA was evaluated for the treatment of acute lymphoblastic leukemia in elderly patients in a phase II study [31]. The dose of 100 IU/kg was found to show the best safety and efficacy profile for these elderly patients. However, median overall survival was disappointing in this treatment regimen, being 15.8 and 9.7 months in the 100 and 150 IU/kg cohorts, respectively. Asparaginase-loaded RBCs were also evaluated in a phase I study in patients with pancreatic adenocarcinoma [32] with a reasonable safety profile. Results of a phase IIb study with erythrocyte-containing asparaginase (now named eryaspase) in combination with chemotherapy in second-line treatment of advanced pancreatic cancer were recently published [33]. The study, funded by Erytech Pharma, was conducted using the ERYCAPS® technology. For the entire population, median overall survival in the eryaspase arm was 6.0 months versus 4.4 months in the control arm receiving chemotherapy. Median progression-free survival was 2.0 months in the eryaspase arm and 1.6 months in the control arm. Overall, the incidence of adverse events was similar in the two arms. An open-label, randomized phase III study of eryaspase in combination with chemotherapy compared with chemotherapy alone as second-line treatment in patient with pancreatic cancer is ongoing and recruiting (NCT03665441). The company Erytech Pharma has also planned a phase II trial in triple negative breast cancer (NCT03674242) and in other solid tumors, and a phase II trial in lymphoblastic leukemia.

RBCs for the Delivery of Thymidine Phosphorylase

Accumulation of thymidine and deoxyuridine in human tissues and body fluids, including blood, is a consequence of a complete or partial absence of the enzyme thymidine phosphorylase and results in a fatal autosomal recessive disorder best described as mitochondrial neurogastrointestinal encephalomyopathy or MNGIE (Online Mendelian inheritance in Man #603041, Genome Database accession #9835128). The biochemical consequences of a high concentration of deoxyribonucleosides is an increased concentration of the corresponding triphosphates within mitochondria, leading to multiple deletions, point mutations, and depletion in mitochondrial DNA. Moran et al. [34] reported the first treatment of a MNGIE patient with autologous erythrocyte-encapsulated recombinant thymidine phosphorylase from Escherichia coli . A detailed description of the clinical and biochemical improvements with this treatment was reported by Bax et al. [35], confirming a successful enzyme replacement therapy for the treatment of MNGIE. These conclusions were confirmed in three patients receiving increasing doses of the encapsulated enzyme [36], and a full phase II, multi-center, multiple dose, uncontrolled, open-label trial will investigate the application of erythrocyte-encapsulated thymidine phosphorylase (EE-TP) as an enzyme replacement therapy for MNGIE. Three EE-TP dose levels are planned with patients eventually receiving the dose level that achieves metabolic correction. The study has a 3-month run-in period followed by a 24-month treatment phase [37, 38]. The treatment has received the designation of orphan drug by both the EMA and FDA. At the date of writing (January 2020), this study is not recruiting yet.

RBCs for the Delivery of Phenylalanine Ammonia Lyase

Phenylketonuria (PKU, OMIM 261600) is an inborn error of metabolism caused by inherited mutations in the enzyme phenylalanine hydroxylase converting phenylalanine (Phe) into tyrosine. This deficiency leads to systemic accumulation of Phe, leading to intellectual disabilities and neurocognitive impairments even in early and continuously treated patients on a Phe-restricted diet [39, 40]. An enzyme replacement therapy based on pegvaliase administration (enzyme produced by BioMarin Pharmaceutical Inc.) has recently been approved by the FDA (May 2018) and EMA (April 2019) for adults with Phe ≥ 600 µM. The administered enzyme is a pegylated recombinant Anabaema variabilis phenylalanine ammonia lyase (PAL) to be administered daily. About 60% of treated patients in a phase III clinical trial achieved blood Phe ≤ 360 µM, but adverse reactions and anaphylaxis were associated with this therapy [41]. To overcome these limitations, a RBC-mediated delivery of PAL was investigated in a validated murine model of PKU [42, 43]. These experiments provided evidence that pegylation is not necessary when the recombinant enzyme is administered encapsulated within autologous RBCs. Furthermore, the selected dose was equally effective at all time points investigated, proving that anti-drug antibodies do not inactivate the recombinant PAL once encapsulated into autologous RBCs. Recently, Rubius announced that their first product to be developed would be based on the delivery of PAL expressed into RBCs (RTX-134) [49]. It is worth noting that Rubius’ technology, as described on the company website, is based on CD34+ hematopoietic precursor cells collected by apheresis from a healthy O-negative donor and purified. These precursor cell populations are genetically engineered with a lentiviral vector, or gene cassette, to express PAL within the cells. The cells are then exposed to media in a bioreactor to promote further expansion and differentiation until the nucleus is ejected, resulting in mature reticulocytes to be administered to patients in need. The company announced FDA clearance of the Investigational New Drug application in March 2019 [50]. The primary objective of this study is to evaluate the safety and tolerability of RTX-134 following intravenous administration of a single dose. RTX-134 consists of allogeneic human red cells expressing AvPAL (Anabaena variabilis phenylalanine ammonia lyase). The trial is designed to determine a preliminary dose and inform a dosing schedule that is deemed safe, tolerable, and potentially effective. Four dose levels are planned and additional dose levels may be explored. Following administration, subjects will be monitored until 28 days after last detection of RTX-134. Detection of RTX-134 will be evaluated using multiple pharmacokinetic and pharmacodynamic assessments including measurement of trans-cinnamic acid (tCA). The number of participants was originally planned to be 12 and the study was due to start in September 2019. However, currently, based on company disclosure, only the first patient has been treated, thus delays are expected in the reading of the clinical data1.

Preclinical Pipeline

The two companies with the most advanced clinical programs (EryDel and Erytech) have a robust pipeline with leading products and developments in additional conditions and new therapeutics. Rubius has also announced a strong pipeline with some preclinical data published. Of interest, due to space limitation, we will address readers to several reviews that have summarized the potential clinical developments of several different candidates already tested in suitable animal models [4448]. The envisaged applications cover many different areas including delivery of small chemical entities, therapeutic proteins, enzymes, immune modulators, and contrasting agents for diagnostic applications.

Concluding Remarks

The technology based on the use of RBCs for the delivery of therapeutic and/or diagnostic agents has strongly evolved from a research field to advanced clinical development programs. Some companies are leading the clinical applications with products currently in phase III trials and robust pipelines. The modalities selected by each company for the production of the modified RBCs are different. While Rubius’ technology is mainly based on the genetic engineering of precursor erythroid cells to be modified by viral vectors, expanded ex vivo and differentiated in cell bioreactors before infusion in patients in need, Erytech Pharma is producing the modified RBCs in dedicated cell facilities starting from blood donations obtained by immune-compatible donors. In contrast, EryDel processes autologous blood at the bedside of the patient. In other words, the patient provides a small amount of blood (i.e., about 50 mL in most studies), which is processed in a dedicated automated system (comprising a drug and medical devices) by a patented technology and re-infused into the original donor within 1.5–2 h from the time of blood collection. Thus, each patient receives their own blood once it is processed by the encapsulation of drugs or other therapeutic agents. This approach is repeated monthly and does not require a centralized cell factory and distribution logistics.

The regulatory authorities in the US and Europe have been involved in several steps of EryDel process development. In addition, clearance of all Investigational New Drug Applications (see Table 2) has greatly contributed to defining the main regulatory requirements in terms of safety and efficacy for the benefit of patients in need. EryDel has shown that transformed RBCs have a 24-h survival and a half-life in circulation that conforms to the FDA criteria for the approval of blood transfusion products [14]. Erytech, based on asparaginase activity [30], has shown that the entrapped asparaginase is detectable in circulation for about 1 month. Procedure-related adverse events of RBC-mediated delivery have not been observed in hundreds of administrations in adults and children. In conclusion, the enormous efforts that a large number of researchers have made in advancing our knowledge of the biology, physiology, immunology, and biochemistry of RBCs is finally contributing to the development of new therapeutics for unmet medical needs, frequently in the area of rare diseases. This great effort is now in the last phase of clinical development for a few products, and this is also thanks to a number of investors and patient associations that have joined their efforts to push this fascinating technology forward to new therapeutic breakthroughs.

Table 2
Clinical trials considered in this article related to the use of red blood cells loaded with therapeutic agents
Study titlePostedLink and identifierStatus
Dexamethasone 21-phosphate
Intra-Erythrocyte Dexamethasone Sodium Phosphate in Ataxia Telangiectasia Patients (IEDAT01)2010


EryDex Pharmacokinetics in Healthy Volunteers (EryDex)2013


Recovery and Survival of EryDex in Non-patient Volunteers2015


EDS in Ataxia Telangiectasia Patients (ATTeST)2016


Open-label, Long-term, Extension Treatment Using Intra-Erythrocyte Dexamethasone Sodium Phosphate in Patients With Ataxia Telangiectasia Who Participated in the IEDAT-02-2015 Study (OLE-IEDAT)2018


Administration of Allogenic Red Blood Cells Loaded L-asparaginase in Cases of Relapse of Acute Lymphoblastic Leukaemia (GRASPALL)2008


GRASPA (Erythrocytes Encapsulating L-asparaginase) in Patients With Relapse of Acute Lymphoblastic Leukemia (GRASPIVOTALL)2012


Administration of GRASPA (Suspension of Erythrocytes Encapsulating L-asparaginase) in Elderly Patients With First Line Acute Lymphoblastic Leukemia2012


Administration of GRASPA (Suspension of Erythrocytes Encapsulating L-asparaginase) in Patients With Pancreatic Cancer2012


GRASPA Treatment for Patients With Acute Myeloblastic Leukemia (ENFORCE)2013


Efficacy and Safety of L-asparaginase Encapsulated in RBC Combined With Gemcitabine or FOLFOX in 2nd Line for Progressive Metastatic Pancreatic Carcinoma2014


Study of Eryaspase in Combination With Chemotherapy Versus Chemotherapy Alone as 2nd-Line Treatment in PDAC (Trybeca-1)2018


Study of Eryaspase in Combination With Chemotherapy Versus Chemotherapy Alone as 1st Line Treatment of TNBC (TRYbeCA-2)2018


Thymidine phosphorylase
Trial of Erythrocyte Encapsulated Thymidine Phosphorylase In Mitochondrial Neurogastrointestinal Encephalomyopathy (TEETPIM)2019


Not yet recruiting
Safety and Tolerability of RTX-134 in Adults With Phenylketonuria2019




1 On March 12, 2020 Rubius Therapeutics announced in a press release that “the first patient was dosed in the Phase 1b PKU clinical trial of RTX-134 in January 2020. While there were no reported adverse events and RTX-134 administration was well tolerated, the results from the first patient were uninterpretable possibly due, in part, to the low dose of cells administered and the sensitivity of the flow cytometry assay used to detect circulating cells. As a result of the deprioritization, the current Phase 1b clinical trial in PKU will be discontinued."

Compliance with Ethical Standards


This work was partially supported by Horizon 2020 IEDAT Grant n. 667946.

Conflicts of interest

Mauro Magnani and Luigia Rossi hold shares in EryDel SpA, a company with interests in the technology of RBC-based drug delivery. The other authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.



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Rubius Therapeutics Announces FDA Clearance of Investigational New Drug Application for First-Ever Red Cell Therapeutic, RTX-134, for Treatment of Phenylketonuria (press release). Rubius Therapeutics, Inc., 11 Mar 2019. Accessed 3 Mar 2020. Developments and Clinical Progress in Drug-Loaded Red Blood Cell Technologies&author=Luigia Rossi,Francesca Pierigè,Mattia Paolo Aliano,Mauro Magnani,&keyword=&subject=Leading Article,