ResearchPad - academicsubjects-med00773 https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Prenatal low-dose methyltestosterone, but not dihydrotestosterone, treatment induces penile formation in female mice and guinea pigs<a href="#afn1"><sup>†</sup></a>]]> https://www.researchpad.co/article/elastic_article_16313 Low-dose methyltestosterone treatment is sufficient to induce tubular urethral formation in female mice and guinea pigs through cellular processes similar to those of males, while same dose of dihydrotestosterone leads to female hypospadias in mice.

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<![CDATA[Sequence comparisons of cytochrome P450 aromatases from Australian animals predict differences in enzymatic activity and/or efficiency<a href="#fn1"><sup>†</sup></a>]]> https://www.researchpad.co/article/elastic_article_16312 Aromatase (P450arom, CYP19A1) is the terminal enzyme in the synthesis of the steroid hormone family of estrogens. Not surprisingly, this enzyme has structural similarities between the limited number of species studied thus far. This study examined the structure of aromatases from four diverse Australian species including a marsupial (tammar wallaby; Macropus eugenii), monotreme (platypus; Ornithorhynchus anatinus), ratite (emu; Dromaius novaehollandiae) and lizard (bearded dragon; Pogona vitticeps). We successfully built homology models for each species, using the only crystallographically determined structure available, human aromatase. The amino acid sequences showed high amino acid sequence identity to the human aromatase: wallaby 81%, platypus 73%, emu 75% and bearded dragon at 74%. The overall structure was highly conserved among the five species, although there were non-secondary structures (loops and bends) that were variable and flexible that may result in some differences in catalytic activity. At the N-terminal regions, there were deletions and variations that suggest that functional distinctions may be found. We found that the active sites of all these proteins were identical, except for a slight variation in the emu. The electrostatic potential across the surfaces of these aromatases highlighted likely variations to the protein-protein interactions of these enzymes with both redox partner cytochrome P450 reductase and possibly homodimerization in the case of the platypus, which has been postulated for the human aromatase enzyme. Given the high natural selection pressures on reproductive strategies, the relatively high degree of conservation of aromatase sequence and structure across species suggests that there is biochemically very little scope for changes to have evolved without the loss of enzyme activity.

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<![CDATA[Characteristics of the endometrium in menstruating species: lessons learned from the animal kingdom<a href="#afn1"><sup>†</sup></a>]]> https://www.researchpad.co/article/elastic_article_16311 A summary of the menstrual cycle and endometrial characteristics of human-like menstruating species.

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