ResearchPad - carbon-dioxide https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Stereoscopic optimization of industrial structure of the equipment manufacturing industry from the perspective of collaborative emissions reduction: Evidence from China]]> https://www.researchpad.co/article/elastic_article_11230 Equipment manufacturing industry is one of the major industries of the Chinese economy. Previous researches have revealed that the industry has dilemmas of unreasonable industrial structure and high pollution. Using the data of 30 provinces in 2006-2015 in China, this study calculated a comprehensive pollution indicator when estimating the possible pollution reduction brought by the optimization of industrial structure and then evaluated the reasonable level of capital allocation of provinces and industries by using the methods of nonlinear programming and stochastic frontier method. Under the target of collaborative emission reduction, the results show that the optimized output of China’s equipment manufacturing industry could be increased by 5.42%, the energy intensity could be reduced by about 10.4%, and the comprehensive emission intensity could be reduced by about 7.47%. Due to the industry heterogeneity and regional heterogeneity, industrial capacity should be transferred between industries and regions. Since the capital investment in the equipment manufacturing industry is significantly mismatched between industries and regions, the capital allocation of provincial industries in China needs to be adjusted properly. This study provides theoretically and practically reference for collaborative pollution reduction, industry restructure, spatial layout and capital investment, which contributes to achieving the stereoscopic optimization of equipment manufacturing industry.

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<![CDATA[Do parents counter-balance the carbon emissions of their children?]]> https://www.researchpad.co/article/Nea582d41-f072-4a93-882b-8bb6cca64243

It is well understood that adding to the population increases CO2 emissions. At the same time, having children is a transformative experience, such that it might profoundly change adult (i.e., parents’) preferences and consumption. How it might change is, however, unknown. Depending on if becoming a parent makes a person “greener” or “browner,” parents may either balance or exacerbate the added CO2 emissions from their children. Parents might think more about the future, compared to childless adults, including risks posed to their children from environmental events like climate change. But parenthood also adds needs and more intensive competition on your scarce time. Carbon-intensive goods can add convenience and help save time, e.g., driving may facilitate being in more places in one day, compared to public transportation or biking. Pre-prepared food that contain red meat may save time and satisfy more household preferences, relative to vegetarian food. We provide the first rigorous test of whether parents are greener or browner than other adults. We create a unique dataset by combining detailed micro data on household expenditures of all expenditure groups particularly important for CO2 emissions (transportation, food, and heating/electricity) with CO2 emissions, and compare emissions from Swedish adults with and without children. We find that parents emit more CO2 than childless adults. Only a small fraction of adults permanently choose not to have children, which means any meaningful self-selection into parenthood based on green preferences is unlikely. Our findings suggest that having children might increase CO2 emissions both by adding to the population and by increasing CO2 emissions from those choosing to have children.

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<![CDATA[Double Phosphinoboration of CO 2 : A Facile Route to Diphospha‐Ureas]]> https://www.researchpad.co/article/Ncf2e152a-d08d-46cf-a701-b0202fe940a8

Abstract

The reactions of CO2 with a series of phosphinoboranes, including R2PBpin (R=Ph, tBu; pin=pinacol), R2PBMes2 (R=Ph, tBu; Mes=2,4,6‐Me3‐C6H2), and R2PBcat (R=Ph, tBu, Mes; cat=catechol) are described. Although R2PBpin and R2PBMes2 afford products of the form R2PCO2Bpin (R=Ph 1, tBu 4) and R2PCO2BMes2 (R=Ph 2, tBu 3), respectively, R2PBcat lead to further reaction affording the diphospha‐ureas, (R2P)2CO (R=Ph 5, tBu 6, Mes 7), together with O(Bcat)2. Computational studies provide insight into the mechanism, revealing an intermediate derived from double phosphinoboration of CO2.

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<![CDATA[How elevated CO2 affects our nutrition in rice, and how we can deal with it]]> https://www.researchpad.co/article/5c8823f8d5eed0c48463945d

Increased concentrations of atmospheric CO2 are predicted to reduce the content of essential elements such as protein, zinc, and iron in C3 grains and legumes, threatening the nutrition of billions of people in the next 50 years. However, this prediction has mostly been limited to grain crops, and moreover, we have little information about either the underlying mechanism or an effective intervention to mitigate these reductions. Here, we present a broader picture of the reductions in elemental content among crops grown under elevated CO2 concentration. By using a new approach, flow analysis of elements, we show that lower absorption and/or translocation to grains is a key factor underlying such elemental changes. On the basis of these findings, we propose two effective interventions—namely, growing C4 instead of C3 crops, and genetic improvements—to minimize the elemental changes in crops, and thereby avoid an impairment of human nutrition under conditions of elevated CO2.

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<![CDATA[Increasing atmospheric CO2 and canopy temperature induces anatomical and physiological changes in leaves of the C4 forage species Panicum maximum]]> https://www.researchpad.co/article/5c75ac6cd5eed0c484d08750

Changes in leaf anatomy and ultrastructure are associated with physiological performance in the context of plant adaptations to climate change. In this study, we investigated the isolated and combined effects of elevated atmospheric CO2 concentration ([CO2]) up to 600 μmol mol-1 (eC) and elevated temperature (eT) to 2°C more than the ambient canopy temperature on the ultrastructure, leaf anatomy, and physiology of Panicum maximum Jacq. grown under field conditions using combined free-air carbon dioxide enrichment (FACE) and temperature free-air controlled enhancement (T-FACE) systems. Plants grown under eC showed reduced stomatal density, stomatal index, stomatal conductance (gs), and leaf transpiration rate (E), increased soil-water content (SWC) conservation and adaxial epidermis thickness were also observed. The net photosynthesis rate (A) and intrinsic water-use efficiency (iWUE) were enhanced by 25% and 71%, respectively, with a concomitant increase in the size of starch grains in bundle sheath cells. Under air warming, we observed an increase in the thickness of the adaxial cuticle and a decrease in the leaf thickness, size of vascular bundles and bulliform cells, and starch content. Under eCeT, air warming offset the eC effects on SWC and E, and no interactions between [CO2] and temperature for leaf anatomy were observed. Elevated [CO2] exerted more effects on external characteristics, such as the epidermis anatomy and leaf gas exchange, while air warming affected mainly the leaf structure. We conclude that differential anatomical and physiological adjustments contributed to the acclimation of P. maximum growing under elevated [CO2] and air warming, improving the leaf biomass production under these conditions.

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<![CDATA[Removal of hydrogen sulfide from a biogas mimic by using impregnated activated carbon adsorbent]]> https://www.researchpad.co/article/5c6c75e0d5eed0c4843d03aa

Adsorption technology has led to the development of promising techniques to purify biogas, i.e., biomethane or biohydrogen. Such techniques mainly depend on the adsorbent ability and operating parameters. This research focused on adsorption technology for upgrading biogas technique by developing a novel adsorbent. The commercial coconut shell activated carbon (CAC) and two types of gases (H2S/N2 and H2S/N2/CO2) were used. CAC was modified by copper sulfate (CuSO4), zinc acetate (ZnAc2), potassium hydroxide (KOH), potassium iodide (KI), and sodium carbonate (Na2CO3) on their surface to increase the selectivity of H2S removal. Commercial H2S adsorbents were soaked in 7 wt.% of impregnated solution for 30 min before drying at 120°C for 24 h. The synthesized adsorbent’s physical and chemical properties, including surface morphology, porosity, and structures, were characterized by SEM-EDX, FTIR, XRD, TGA, and BET analyses. For real applications, the modified adsorbents were used in a real-time 0.85 L single-column adsorber unit. The operating parameters for the H2S adsorption in the adsorber unit varied in L/D ratio (0.5–2.5) and feed flow rate (1.5–5.5 L/min) where, also equivalent with a gas hourly space velocity, GHSV (212.4–780.0 hour-1) used. The performances of H2S adsorption were then compared with those of the best adsorbent that can be used for further investigation. Characterization results revealed that the impregnated solution homogeneously covered the adsorbent surface, morphology, and properties (i.e., crystallinity and surface area). BET analysis further shows that the modified adsorbents surface area decreased by up to 96%. Hence, ZnAc2–CAC clarify as the best adsorption capacity ranging within 1.3–1.7 mg H2S/g, whereby the studied extended to adsorption-desorption cycle.

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<![CDATA[Predicting the direct and indirect impacts of climate change on malaria in coastal Kenya]]> https://www.researchpad.co/article/5c648cdfd5eed0c484c819a9

Background

The transmission of malaria is highly variable and depends on a range of climatic and anthropogenic factors. This study investigates the combined, i.e. direct and indirect, impacts of climate change on the dynamics of malaria through modifications in: (i) the sporogonic cycle of Plasmodium induced by air temperature increase, and (ii) the life cycle of Anopheles vector triggered by changes in natural breeding habitat arising from the altered moisture dynamics resulting from acclimation responses of vegetation under climate change. The study is performed for a rural region in Kilifi county, Kenya.

Methods and findings

We use a stochastic lattice-based malaria (SLIM) model to make predictions of changes in Anopheles vector abundance, the life cycle of Plasmodium parasites, and thus malaria transmission under projected climate change in the study region. SLIM incorporates a nonlinear temperature-dependence of malaria parasite development to estimate the extrinsic incubation period of Plasmodium. It is also linked with a spatially distributed eco-hydrologic modeling framework to capture the impacts of climate change on soil moisture dynamics, which served as a key determinant for the formation and persistence of mosquito larval habitats on the land surface. Malaria incidence data collected from 2008 to 2013 is used for SLIM model validation. Projections of climate change and human population for the region are used to run the models for prediction scenarios.

Under elevated atmospheric CO2 concentration ([CO2]) only, modeled results reveal wetter soil moisture in the root zone due to the suppression of transpiration from vegetation acclimation, which increases the abundance of Anopheles vectors and the risk of malaria. When air temperature increases are also considered along with elevated [CO2], the life cycle of Anopheles vector and the extrinsic incubation period of Plasmodium parasites are shortened nonlinearly. However, the reduction of soil moisture resulting from higher evapotranspiration due to air temperature increase also reduces the larval habitats of the vector. Our findings show the complicated role of vegetation acclimation under elevated [CO2] on malaria dynamics and indicate an indirect but ignored impact of air temperature increase on malaria transmission through reduction in larval habitats and vector density.

Conclusions

Vegetation acclimation triggered by elevated [CO2] under climate change increases the risk of malaria. In addition, air temperature increase under climate change has opposing effects on mosquito larval habitats and the life cycles of both Anopheles vectors and Plasmodium parasites. The indirect impacts of temperature change on soil moisture dynamics are significant and should be weighed together with the direct effects of temperature change on the life cycles of mosquitoes and parasites for future malaria prediction and control.

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<![CDATA[Nitrogen gas produces less behavioural and neurophysiological excitation than carbon dioxide in mice undergoing euthanasia]]> https://www.researchpad.co/article/5c5ca2bcd5eed0c48441e9d5

Carbon dioxide (CO2) is one of the most commonly used gas euthanasia agents in mice, despite reports of aversion and nociception. Inert gases such as nitrogen (N2) may be a viable alternative to carbon dioxide. Here we compared behavioural and electrophysiological reactions to CO2 or N2 at either slow fill or rapid fill in C57Bl/6 mice undergoing gas euthanasia. We found that mice euthanised with CO2 increased locomotor activity compared to baseline, whereas mice exposed to N2 decreased locomotion. Furthermore, mice exposed to CO2 showed significantly more vertical jumps and freezing episodes than mice exposed to N2. We further found that CO2 exposure resulted in increased theta:delta of the EEG, a measure of excitation, whereas the N2 decreased theta:delta. Differences in responses were not oxygen-concentration dependent. Taken together, these results demonstrate that CO2 increases both behavioural and electrophysiological excitation as well as producing a fear response, whereas N2 reduces behavioural activity and central neurological depression and may be less aversive although still produces a fear response. Further studies are required to evaluate N2 as a suitable euthanasia agent for mice.

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<![CDATA[Fire, CO2, and climate effects on modeled vegetation and carbon dynamics in western Oregon and Washington]]> https://www.researchpad.co/article/5c57e67cd5eed0c484ef33e4

To develop effective long-term strategies, natural resource managers need to account for the projected effects of climate change as well as the uncertainty inherent in those projections. Vegetation models are one important source of projected climate effects. We explore results and associated uncertainties from the MC2 Dynamic Global Vegetation Model for the Pacific Northwest west of the Cascade crest. We compare model results for vegetation cover and carbon dynamics over the period 1895–2100 assuming: 1) unlimited wildfire ignitions versus stochastic ignitions, 2) no fire, and 3) a moderate CO2 fertilization effect versus no CO2 fertilization effect. Carbon stocks decline in all scenarios, except without fire and with a moderate CO2 fertilization effect. The greatest carbon stock loss, approximately 23% of historical levels, occurs with unlimited ignitions and no CO2 fertilization effect. With stochastic ignitions and a CO2 fertilization effect, carbon stocks are more stable than with unlimited ignitions. For all scenarios, the dominant vegetation type shifts from pure conifer to mixed forest, indicating that vegetation cover change is driven solely by climate and that significant mortality and vegetation shifts are likely through the 21st century regardless of fire regime changes.

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<![CDATA[Effects of long-term fertilization on soil organic carbon mineralization and microbial community structure]]> https://www.researchpad.co/article/5c57e66bd5eed0c484ef30c6

Soil microorganisms play a pivotal role in carbon mineralization and their diversity is crucial to the function of soil ecosystems. However, the effects of long-term fertilization on microbial-mediated carbon mineralization are poorly understood. To identify the relative roles of microbes in carbon mineralization of yellow paddies, we investigated the long-term fertilization effects on soil properties and microbial communities and their relationships with carbon mineralization. The treatments included: no fertilization (CK), chemical fertilizer (NPK), organic fertilizer (M), and constant organic-inorganic fertilizer (MNPK). NPK treatment significantly increased soil water content (WC), while M and MNPK treatments significantly increased the content of soil organic carbon (SOC), total nitrogen (TN), soil microbial biomass carbon (SMBC), soil microbial biomass nitrogen (SMBN), and WC. Strong increases in CO2 emissions, potential mineralized carbon, and turnover rate constant were observed in both organic-fertilizer treatments (M and MNPK), relative to the CK treatment. These changes in soil properties can be attributed to the variation in microbial communities. NPK treatment had no significant effect. Different fertilization treatments changed soil microbial community; SOC and SMBN were the most important contributors to the variance in microbial community composition. The variations in community composition did not significant influence carbon mineralization; however, carbon mineralization was significantly influenced by the abundance of several non-dominant bacteria. The results suggest that SOC, SMBN, and non-dominant bacteria (Gemmatimonadetes and Latescibacteria), have a close relationship to carbon mineralization, and should be preferentially considered in predicting carbon mineralization under long-term fertilization.

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<![CDATA[Plant biomass and soil organic carbon are main factors influencing dry-season ecosystem carbon rates in the coastal zone of the Yellow River Delta]]> https://www.researchpad.co/article/5c466518d5eed0c4845175f4

Coastal wetlands are considered as a significant sink of global carbon due to their tremendous organic carbon storage. Coastal CO2 and CH4 flux rates play an important role in regulating atmospheric CO2 and CH4 concentrations. However, the relative contributions of vegetation, soil properties, and spatial structure on dry-season ecosystem carbon (C) rates (net ecosystem CO2 exchange, NEE; ecosystem respiration, ER; gross ecosystem productivity, GEP; and CH4) remain unclear at a regional scale. Here, we compared dry-season ecosystem C rates, plant, and soil properties across three vegetation types from 13 locations at a regional scale in the Yellow River Delta (YRD). The results showed that the Phragmites australis stand had the greatest NEE (-1365.4 μmol m-2 s-1), ER (660.2 μmol m-2 s-1), GEP (-2025.5 μmol m-2 s-1) and acted as a CH4 source (0.27 μmol m-2 s-1), whereas the Suaeda heteroptera and Tamarix chinensis stands uptook CH4 (-0.02 to -0.12 μmol m-2 s-1). Stepwise multiple regression analysis demonstrated that plant biomass was the main factor explaining all of the investigated carbon rates (GEP, ER, NEE, and CH4); while soil organic carbon was shown to be the most important for explaining the variability in the processes of carbon release to the atmosphere, i.e., ER and CH4. Variation partitioning results showed that vegetation and soil properties played equally important roles in shaping the pattern of C rates in the YRD. These results provide a better understanding of the link between ecosystem C rates and environmental drivers, and provide a framework to predict regional-scale ecosystem C fluxes under future climate change.

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<![CDATA[Individual and combined effects of low dissolved oxygen and low pH on survival of early stage larval blue crabs, Callinectes sapidus]]> https://www.researchpad.co/article/5c141eded5eed0c484d289a2

A large number of coastal ecosystems globally are subjected to concurrent hypoxic and acidified conditions that will likely intensify and expand with continued climate change. In temperate regions, the spawning of many important organisms including the Atlantic blue crab Callinectes sapidus occurs during the summer months when the severity of coastal hypoxia and acidification is the greatest. While the blue crab earliest larval stage can be exposed to co-occurring hypoxia and acidification observed in many coastal ecosystems, the effects of these concurrent stressors on larval blue crab survival is unknown. This study investigated the individual and combined consequences of low dissolved oxygen (DO) and low pH on blue crab larvae survival through a series of short-term experiments. During 14-day experiments with moderately hypoxic conditions (117–127 μM O2 or 3.74–4.06 mg L-1) and acidified conditions (pH on total scale of 7.16–7.33), low DO and low pH individually and significantly reduced larval survival by 60% and 49%, respectively, with the combination of stressors reducing survival by 87% compared to the control treatment (210–269 μM O2 or 6.72–8.61 mg L-1, 7.91–7.94 DO and pH, respectively). During 4-day experiments with lower DO levels (68–83 μM O2 or 2.18–2.62 mg L-1) and comparable pH levels of 7.29–7.39, low DO individually reduced survival by >90% compared to the control (261–267 μM O2 or 8.35–8.54 mg L-1, 7.92–7.97 DO and pH, respectively), whereas low pH had no effect and there was no interaction between stressors. Over a 4-day period, the DO threshold at which 50% of the larval blue crab population died (LC50) was 121 μM O2 (3.86 mgL-1). In 14-day experiments, the DO and pH effects were additive, yielding survival rates lower than the individual treatments, and significantly correlated with DO and pH concentrations. Collectively, these findings indicate that blue crab sensitivity to both low DO and low pH are acute within the larval stage, depend on the intensity and duration of exposure, and leads to mortality, thereby potentially contributing to the interannual variability and possible regional declines of this fishery.

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<![CDATA[Spiroergometric measurements under increased inspiratory oxygen concentration (FIO2)—Putting the Haldane transformation to the test]]> https://www.researchpad.co/article/5c1ab87ad5eed0c484028333

Spiroergometric measurements of persons who require oxygen insufflation due to illness can be performed under conditions of increased inspiratory oxygen concentration (FIO2). This increase in FIO2, however, often leads to errors in the calculation of oxygen consumption (V˙O2). These inconsistencies are due to the application of the Haldane Transformation (HT), an otherwise indispensable correction factor in the calculation of V˙O2 that becomes inaccurate at higher FIO2 concentrations. A possible solution to this problem could be the use of the ‘Eschenbacher transformation’ (ET) as an alternative correction factor. This study examines the concentration of FIO2 at which the HT and the ET are valid, providing plausible data of oxygen consumption corresponding to the wattage achieved during cycle ergometry. Ten healthy volunteers underwent spiroergometric testing under standard conditions (FIO2 = 20.9%), as well as at FIO2 = 40% and 80%. When compared with the predicted values of V˙O2, as calculated according to Wasserman et al. (2012), the data obtained show that both the HT and ET are valid under normal conditions and at an increased FIO2 of 40%. At FIO2 concentrations of 80%, however, the V˙O2 values provided by the HT begin to lose plausibility, whereas the ET continues to provide credible results. We conclude that the use of the ET in place of the HT in spiroergometric measurements with increased FIO2 allows a reliable evaluation of stress tests in patients requiring high doses of supplemental oxygen.

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<![CDATA[Doctors’ perceptions of the impact of upfront point-of-care testing in the emergency department]]> https://www.researchpad.co/article/5c1c0b08d5eed0c4844272a0

Objectives

Special investigations (e.g. blood tests, electrocardiograms, x-rays) play an integral role in patient management in the emergency department (ED). Having results immediately available prior to assessing a patient may lead to improved efficiency. This could be instituted by utilizing point-of-care (POC) testing with an alternative ED workflow, but the implementation would be dependent on acceptance by the end-users. The aim of this study was to assess doctors’ perceptions of POC testing in the ED when the normal treatment pathway was modified to use upfront POC tests performed prior to doctor evaluation in an effort to decrease treatment times.

Methods

A prospective, randomized, controlled trial was performed in the ED where medical patients received either the normal ED workflow pathway or one of the enhanced workflow pathways with POC tests in various combinations prior to doctor evaluation. At the end of the study period, doctors were invited to participate in an anonymous survey to gauge their opinions on the implementation of the early POC testing.

Results

Overall, the doctors surveyed were very satisfied with use of upfront POC in the ED. One hundred per cent of the 28 doctors surveyed found it helpful to assess patients who already had test results available and would want it to be permanently available. Normalized satisfaction scores were more favorable for combinations of 3 or more tests (0.7–1.0) as opposed to combinations with 2 or less tests (0.3–0.7). There was a preference for combinations that included comprehensive blood results.

Conclusion

The implementation of workflow changes to assist doctors in the ED can potentially make them more productive. End-user buy-in is essential in order for the change to be successful. Upfront, protocolised, POC testing is a low-input, high-yield intervention that decreased treatment time and satisfied doctors.

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<![CDATA[Asymmetric relationship of urbanization and CO2 emissions in less developed countries]]> https://www.researchpad.co/article/5c141f15d5eed0c484d29959

Understanding the relationship between carbon dioxide (CO2) emissions and the urbanization of national populations has been a key concern for environmental scholars for several decades. Although sophisticated modeling techniques have been developed to explore the connection between increases in urban populations and CO2 emissions, none has attempted to assess whether declines in urbanization have an effect on emissions that is not symmetrical with that of growth in urbanization. The present study uses panel data on CO2 emissions and the percentage of individuals living in urban areas, as well as a variety of other structural factors, for less-developed countries from 1960–2010, to empirically assess whether the effect of growth in urban populations on emissions is symmetrical with the effect of decline. Findings indicate that the effect of growth/decline in urban populations on CO2 emissions is asymmetrical, where a decline in urbanization reduces emissions to a much greater degree than urbanization increases emissions. We hypothesize that this is at least in part because deurbanization is connected with disruptions to the production and distribution of goods and services and/or access to electricity and other energy sources. Our finding suggests that not only the absolute level of urbanization of nations matters for emissions, but also how the patterns of migration between rural and urban areas change over time. Future research should be mindful of the processes behind deurbanization when exploring socioeconomic drivers of CO2 emissions.

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<![CDATA[Relationship between hemodynamic parameters and severity of ischemia-induced left ventricular wall thickening during cardiopulmonary resuscitation of consistent quality]]> https://www.researchpad.co/article/5c0841e9d5eed0c484fcb218

Ischemia-induced left ventricular (LV) wall thickening compromises the hemodynamic effectiveness of cardiopulmonary resuscitation (CPR). However, accurate assessment of the severity of ischemia-induced LV wall thickening during CPR is challenging. We investigated, in a swine model, whether hemodynamic parameters, including end-tidal carbon dioxide (ETCO2) level, are linearly associated with the severity of ischemia-induced LV wall thickening during CPR of consistent quality. We retrospectively analyzed 96 datasets for ETCO2 level, arterial pressure, LV wall thickness, and the percent of measured end-diastolic volume (%EDV) relative to EDV at the onset of ventricular fibrillation from eight pigs. Animals underwent advanced cardiovascular life support based on resuscitation guidelines. During CPR, LV wall thickness progressively increased while %EDV progressively decreased. Systolic and diastolic arterial pressure and ETCO2 level were significantly correlated with LV wall thickness and %EDV. Linear mixed effect models revealed that, after adjustment for significant covariates, systolic and diastolic arterial pressure were not associated with LV wall thickness or %EDV. ETCO2 level had a significant linear relationship with %EDV (P = 0.004). However, it could explain only 28.2% of the total variance of %EDV in our model. In conclusion, none of the hemodynamic parameters examined in this study appeared to provide sufficient information on the severity of ischemia-induced LV wall thickening.

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<![CDATA[Effect of elevated CO2 and spectral quality on whole plant gas exchange patterns in tomatoes]]> https://www.researchpad.co/article/5bd2325040307c60de5e9972

In controlled environment plant production facilities, elevating either light or CO2 levels generally has led to increased biomass and yield due to enhanced canopy photosynthesis. Today, advancements in light-emitting diodes (LEDs) have made this technology a viable option for both supplementary lighting in greenhouses and a sole lighting source in controlled environment chambers. Our study used tomato plants grown under both ambient CO2 (AC) and elevated CO2 (EC) conditions then exposed them to various CO2 and lighting treatments during both whole plant and leaf level measurements. Plants grown under EC reached the first flower developmental stage 8 days sooner and were approximately 15cm taller than those grown under AC. However, under AC plants had more leaf area while their dry weights were similar. Of note, under EC chlorophyll a and b were lower, as were carotenoids per unit leaf area. Whole plant analyses, under all CO2 challenges, showed that plants exposed to high-pressure sodium (HPS), red-blue LED, and red-white LED had similar photosynthesis, respiration, and daily carbon gain. Under different light qualities, day-time transpiration rates were similar among CO2 conditions. Day-time water-use efficiency (WUE) was higher in plants grown and exposed to EC. Similarly, WUE of plants grown under AC but exposed to short-term elevated CO2 conditions was higher than those grown and tested under AC during all light treatments. Under all CO2 conditions, plants exposed to red-white and red-blue LEDs had lower WUE than those exposed to HPS lighting. Assessing alterations due to CO2 and light quality on a whole plant basis, not merely on an individual leaf basis, furthers our understanding of the interactions between these two parameters during controlled environment production. Principle component analyses of both whole plant and leaf data indicates that increasing CO2 supply has a more dramatic effect on photosynthesis and WUE than on transpiration.

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<![CDATA[Paxillin phosphorylation at serine 273 and its effects on Rac, Rho and adhesion dynamics]]> https://www.researchpad.co/article/5b4a1919463d7e428027f887

Focal adhesions are protein complexes that anchor cells to the extracellular matrix. During migration, the growth and disassembly of these structures are spatiotemporally regulated, with new adhesions forming at the leading edge of the cell and mature adhesions disassembling at the rear. Signalling proteins and structural cytoskeletal components tightly regulate adhesion dynamics. Paxillin, an adaptor protein within adhesions, is one of these proteins. Its phosphorylation at serine 273 (S273) is crucial for maintaining fast adhesion assembly and disassembly. Paxillin is known to bind to a GIT1-βPIX-PAK1 complex, which increases the local activation of the small GTPase Rac. To understand quantitatively the behaviour of this system and how it relates to adhesion assembly/disassembly, we developed a mathematical model describing the dynamics of the small GTPases Rac and Rho as determined by paxillin S273 phosphorylation. Our model revealed that the system possesses bistability, where switching between uninduced (active Rho) and induced (active Rac) states can occur through a change in rate of paxillin phosphorylation or PAK1 activation. The bistable switch is characterized by the presence of memory, minimal change in the levels of active Rac and Rho within the induced and uninduced states, respectively, and the limited regime of monostability associated with the uninduced state. These results were validated experimentally by showing the presence of bimodality in adhesion assembly and disassembly rates, and demonstrating that Rac activity increases after treating Chinese Hamster Ovary cells with okadaic acid (a paxillin phosphatase inhibitor), followed by a modest recovery after 20 min washout. Spatial gradients of phosphorylated paxillin in a reaction-diffusion model gave rise to distinct regions of Rac and Rho activities, resembling polarization of a cell into front and rear. Perturbing several parameters of the model also revealed important insights into how signalling components upstream and downstream of paxillin phosphorylation affect dynamics.

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<![CDATA[Simultaneous quantum yield measurements of carbon uptake and oxygen evolution in microalgal cultures]]> https://www.researchpad.co/article/5b49a28a463d7e0f6c98de32

The photosynthetic quantum yield (Φ), defined as carbon fixed or oxygen evolved per unit of light absorbed, is a fundamental but rarely determined biophysical parameter. A method to estimate Φ for both net carbon uptake and net oxygen evolution simultaneously can provide important insights into energy and mass fluxes. Here we present details for a novel system that allows quantification of carbon fluxes using pH oscillation and simultaneous oxygen fluxes by integration with a membrane inlet mass spectrometer. The pHOS system was validated using Phaeodactylum tricornutum cultured with continuous illumination of 110 μmole quanta m-2 s-1 at 25°C. Furthermore, simultaneous measurements of carbon and oxygen flux using the pHOS-MIMS and photon flux based on spectral absorption were carried out to explore the kinetics of Φ in P. tricornutum during its acclimation from low to high light (110 to 750 μmole quanta m-2 s-1). Comparing results at 0 and 24 hours, we observed strong decreases in cellular chlorophyll a (0.58 to 0.21 pg cell-1), Fv/Fm (0.71 to 0.59) and maximum ΦCO2 (0.019 to 0.004) and ΦO2 (0.028 to 0.007), confirming the transition toward high light acclimation. The Φ time-series indicated a non-synchronized acclimation response between carbon uptake and oxygen evolution, which has been previously inferred based on transcriptomic changes for a similar experimental design with the same diatom that lacked physiological data. The integrated pHOS-MIMS system can provide simultaneous carbon and oxygen measurements accurately, and at the time-resolution required to resolve high-resolution carbon and oxygen physiological dynamics.

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<![CDATA[PLoS Medicine Issue Image | Vol. 15(7) July 2018]]> https://www.researchpad.co/article/5b6d8c4e463d7e2ccdcccfec

Climate change and health: Moving from theory to practice

July's issue of PLOS Medicine includes a special issue on climate change and health. Included in this exciting issue are papers focusing on heatwaves and mortality or morbidity; the impact of air conditioning on greenhouse gas emissions; wildfires and health impacts; health sector contributions to emissions; the effects of increased CO2 on crop nutrients; the occurrence of sewage in urban water supplies as a consequence of rainwater surge; and modelling studies focusing on dengue risk in small island states. The Guest Editors Jonathan Patz (Global Health Institute, University of Wisconsin-Madison) and Madeleine Thomson (International Research Institute for Climate and Society, Columbia University) discuss the papers in their Editorial and reveal how these papers provide an important and compelling advance to the field of climate change and health.

Image Credit: Akuppa John Wigham, Flickr; California National Guard, Flickr

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