ResearchPad - cell-cycle-and-division https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes]]> https://www.researchpad.co/article/Nb6d75dad-2639-4af8-9105-fd776afba33a Accurate chromosome segregation demands efficient capture of microtubules by kinetochores and their conversion to stable bioriented attachments that can congress and then segregate chromosomes. An early event is the shedding of the outermost fibrous corona layer of the kinetochore following microtubule attachment. Centromere protein F (CENP-F) is part of the corona, contains two microtubule-binding domains, and physically associates with dynein motor regulators. Here, we have combined CRISPR gene editing and engineered separation-of-function mutants to define how CENP-F contributes to kinetochore function. We show that the two microtubule-binding domains make distinct contributions to attachment stability and force transduction but are dispensable for chromosome congression. We further identify a specialized domain that functions to limit the dynein-mediated stripping of corona cargoes through a direct interaction with Nde1. This antagonistic activity is crucial for maintaining the required corona composition and ensuring efficient kinetochore biorientation.

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<![CDATA[CDK1 couples proliferation with protein synthesis]]> https://www.researchpad.co/article/N53a3a1ce-9100-42c9-809d-1a3905258ce6

To ensure proper cell growth, the rates of proliferation and protein synthesis are tightly coupled. Haneke et al. demonstrate that the mitotic kinase CDK1 acts as an activator of global protein synthesis during all phases of the cell cycle.

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<![CDATA[Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A]]> https://www.researchpad.co/article/N77ab7992-fe8e-4e0f-a0d8-cc870934c092

Gradients of Aurora A and B kinase activity at the mitotic spindle are crucial for chromosome alignment. Poser et al. show how Aurora A at the spindle poles promotes chromosome congression by activating the chromokinesin KIF4A.

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