ResearchPad - crop-molecular-genetics https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Overexpression of soybean <i>DREB1</i> enhances drought stress tolerance of transgenic wheat in the field]]> https://www.researchpad.co/article/elastic_article_10055 Drought-response-element binding (DREB)-like transcription factors can significantly enhance plant tolerance to water stress. However, most research on DREB-like proteins to date has been conducted in growth chambers or greenhouses, so there is very little evidence available to support their practical use in the field. In this study, we overexpressed GmDREB1 from soybean in two popular wheat varieties and conducted drought-tolerance experiments across a range of years, sites, and drought-stress regimes. We found that the transgenic plants consistently exhibited significant improvements in yield performance and a variety of physiological traits compared with wild-type plants when grown under limited water conditions in the field, for example showing grain yield increases between 4.79–18.43%. Specifically, we found that the transgenic plants had reduced membrane damage and enhanced osmotic adjustment and photosynthetic efficiency compared to the non-transgenic controls. Three enzymes from the biosynthetic pathway of the phytohormone melatonin were up-regulated in the transgenic plants, and external application of melatonin was found to improve drought tolerance. Together, our results demonstrate the utility of transgenic overexpression of GmDREB1 to improve the drought tolerance of wheat in the field.

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<![CDATA[Genetic relationships between spring emergence, canopy phenology, and biomass yield increase the accuracy of genomic prediction in Miscanthus]]> https://www.researchpad.co/article/5c083356d5eed0c484f999b1

Multivariate analysis unveils complex genetic relationships between spring emergence, canopy phenology and biomass yield in Miscanthus, leading to increased response to selection and substantial improvements in genome-wide prediction.

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<![CDATA[Genetic architecture of photosynthesis in Sorghum bicolor under non-stress and cold stress conditions]]> https://www.researchpad.co/article/5c0832d3d5eed0c484f98222

Multiple genomic regions explain variation in the carbon fixation response to non-stress and cold stress in Sorghum bicolor, with some of these regions simultaneously controlling multiple traits.

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<![CDATA[Deciphering the Theobroma cacao self-incompatibility system: from genomics to diagnostic markers for self-compatibility]]> https://www.researchpad.co/article/5c08329ad5eed0c484f97710

Two different mechanisms controlling the Theobroma cacao self-incompatibility system were identified at two separate loci together with candidate genes differentially expressed between self-compatible/incompatible reactions, and diagnostic markers predicting self-compatible varieties were produced.

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<![CDATA[A genome-wide survey with different rapeseed ecotypes uncovers footprints of domestication and breeding]]> https://www.researchpad.co/article/5b69375a463d7e32f55a16aa

In 198 selective sweeps we found functional genes or loci, such as FT and resistance, associated with environmental adaptability and yield-related traits.

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<![CDATA[RNA sequencing of Brassica napus reveals cellular redox control of Sclerotinia infection]]> https://www.researchpad.co/article/5c0832c8d5eed0c484f97f6b

Protection against Sclerotinia sclerotiorum in Brassica napus is mediated via dynamic transcription factor networks and cellular redox homeostasis directly at the site of infection.

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<![CDATA[Vitamin B1 diversity and characterization of biosynthesis genes in cassava]]> https://www.researchpad.co/article/5c08328ed5eed0c484f97552

Vitamin B1 biosynthesis de novo is regulated by duplicated genes and a conserved riboswitch in cassava. Diversity of vitamin B1 levels in cassava leaves negatively correlates with expression of selected vitamin B1 biosynthesis genes.

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