ResearchPad - laboratory-research https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Metformin Prevents H<sub>2</sub>O<sub>2</sub>-Induced Senescence in Human Lens Epithelial B3 Cells]]> https://www.researchpad.co/article/N2cdb302a-ab3c-4a75-9fa2-2d7422a04a74 The primary purpose of this study was to investigate the protective effect of metformin against hydrogen peroxide (H2O2)-induced cellular senescence and to explore the underlying molecular mechanism of lens epithelial cell senescence.Material/MethodsWe used H2O2 to establish senescence in human lens epithelial B3 cells. The cells were exposed to H2O2 for different numbers of days to mimic aging. Senescence was assessed by senescence-associated β-galactosidase staining, and the molecular mechanism was assessed by real-time polymerase chain reaction (RT-PCR) and western blot analysis. The cultured cells were exposed to 150 μM H2O2 for 7 days with or without metformin to detect the underlying molecular mechanism of lens epithelial cell senescence.ResultsThe lens epithelial cells exposed to 150 μM H2O2 for 7 days exhibited senescence. The expression levels of senescence-related markers were increased in H2O2-treated cells. Metformin prevented H2O2-induced cellular senescence in human lens epithelial B3 cells.ConclusionsThese findings suggest that senescence marker expression is increased in the cells exposed to H2O2. Metformin protects human lens epithelial B3 cells from H2O2-induced senescence. ]]> <![CDATA[Antimicrobial Effect of Orthodontic Materials on Cariogenic Bacteria Streptococcus mutans and Lactobacillus acidophilus]]> https://www.researchpad.co/article/N5b016ade-1b8c-4dc5-84ef-7b42e02e9ca9

Background

White spot lesions (WSLs) are a common complication after orthodontic treatment. The aim of this study was to characterize and compare the antimicrobial properties of selenium-containing vs. fluoride-containing orthodontic materials.

Material/Methods

Antibacterial efficacy of orthodontic materials (SeLECT Defense bonding agent, Adhesive agent, Band Cement, Transbond Plus SEP bonding agent, Transbond Plus Adhesive agent, Fuji I Band cement, Fuji Ortho LC Adhesive agent, Ortho Solo Bonding agent, Transbond XT bonding agent, and Transbond XT primer) was tested with the inhibition of 2 bacterial strains: S. mutans (ATCC 10449) and L. acidophilus (ATCC 4356). The antimicrobial efficacy of the materials was measured by agar diffusion test. The diameters of inhibition zones around each disk were measured in millimeters (mm).

Results

Materials containing selenium and fluoride showed significant differences from the negative control (both p<0.001). Orthodontic materials containing fluoride as a potential antimicrobial agent showed larger zones of inhibition in total (9.1±2.6 mm), the selenium group was the second-most effective (4.7±4.9 mm), and the group without any potential antimicrobial agent showed the least antimicrobial effect (0.9±1.0 mm). Materials from the group with no antibacterial agent were not significantly different from the negative control group (p>0.05).

Conclusions

Materials containing selenium carried the most significance when comparing microorganisms with the agent, since they were the only ones showing difference between the 2 microorganisms. They showed statistically significant difference in efficacy against S. mutans, and poor antimicrobial effect against L. acidophilus. These data suggest that orthodontic materials containing selenium might have the potential to prevent WSLs due to their antimicrobial properties.

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<![CDATA[miR-193a-3p Promotes Radio-Resistance of Nasopharyngeal Cancer Cells by Targeting SRSF2 Gene and Hypoxia Signaling Pathway]]> https://www.researchpad.co/article/5c8bfeb1d5eed0c484b291c4

Background

Radio-resistance is an important barrier in nasopharyngeal carcinoma treatment. MicroRNAs are gene expression core regulators in various biological procedures containing cancer radio-resistance. Nevertheless, the clinical association between nasopharyngeal carcinoma and miR-193a-3p/SRSF2 remains unclear.

Material/Methods

We examined the miR-193a-3p level in radio-sensitive CNE-2 and radio-resistant CNE-1 NPC cell lines, and, based on a literature review, predicted SRSF2 to be the target gene of miR-193a-3p. We explored the expression of SRSF2 at protein and mRNA levels by transfecting either miR-193a-3p-mimic or antagomiR. Finally, we performed signaling pathway analysis to assess the possible role of miR-193a-3p/SRSF2 in signaling pathways.

Results

miR-193a-3p promotes NPC radio-resistance, and the SRSF2 gene is the direct target for miR-193a-3p in NPC, and thus is negatively correlated with NPC radio-resistance. The hypoxia signaling pathway activity is strongly affected, and it is possible to use the downstream activity of the SRSF2 gene to show the effect of miR-193a-3p on radio-resistance in NPC cells.

Conclusions

miR-193a-3p mediates promotion of NPC radio-resistance.

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