ResearchPad - letters-to-the-editor https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Roles and challenges of primary care physicians facing a dual outbreak of COVID-19 and dengue in Singapore]]> https://www.researchpad.co/article/elastic_article_12302 <![CDATA[ <i>IL1RL1</i> gene variations are associated with asthma exacerbations in children and adolescents using inhaled corticosteroids]]> https://www.researchpad.co/article/elastic_article_7352 <![CDATA[Gaps between actual initial treatment of anaphylaxis in China and international guidelines: A review and analysis of 819 reported cases]]> https://www.researchpad.co/article/elastic_article_7078 <![CDATA[C3a signaling is not involved in eosinophil migration during experimental allergic lung inflammation in mice]]> https://www.researchpad.co/article/elastic_article_6821 <![CDATA[Effect of C1‐inhibitor in adults with mild asthma: A randomized controlled trial]]> https://www.researchpad.co/article/elastic_article_6793 <![CDATA[Update: Mepolizumab treatment in patients with severe eosinophilic asthma and prior omalizumab use]]> https://www.researchpad.co/article/elastic_article_6734 <![CDATA[The bioinformatics wealth of nations]]> https://www.researchpad.co/article/N5da40a0e-3f32-497d-bfe5-58f85b07ab10 <![CDATA[IN MEMORIAM Christopher Noel Williams December 25, 1935 to December 18, 2019]]> https://www.researchpad.co/article/N8e00ea9b-1007-4567-b5d5-a0117c794dd7 <![CDATA[Association between regional selenium status and reported outcome of COVID-19 cases in China]]> https://www.researchpad.co/article/N2c403ff5-8cc4-428c-839d-ecc59d5d6854 <![CDATA[Response to letter to editor regarding Immunoglobulin G4‐related disease in a dog]]> https://www.researchpad.co/article/N4ddc738e-26a9-470f-a8fa-c49bbdd7d498 ]]> <![CDATA[Letter to the editor regarding Immunoglobulin G4‐related disease in a dog]]> https://www.researchpad.co/article/N1d5ca20c-62dd-4a31-bf02-df83cb6df3bb ]]> <![CDATA[An Outbreak of Severe Acute Respiratory Syndrome in a Nursing Home]]> https://www.researchpad.co/article/N5e64a3c1-dbb3-4845-804c-b421bf3eec4f ]]> <![CDATA[SIMILARITIES OF IMMUNE REACTIONS BETWEEN HEPATITIS C AND SEVERE ACUTE RESPIRATORY SYNDROME-ASSOCIATED CORONAVIRUS INFECTIONS]]> https://www.researchpad.co/article/N4631c5b7-30d9-4826-b35d-26c1c8a20195 ]]> <![CDATA[Re: To KF, Tong JH, Chan PK,et al. Tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: anin-situ hybridization study of fatal cases.J Pathol 2004; 202: 157–163]]> https://www.researchpad.co/article/N16b6d88f-0d2a-4415-8eeb-0cc2f92799c3 ]]> <![CDATA[Public responses to the novel 2019 coronavirus (2019‐nCoV) in Japan: Mental health consequences and target populations]]> https://www.researchpad.co/article/Nc4eb715f-8ee1-45c6-93de-dfea368d371f ]]> <![CDATA[Severe acute respiratory symptoms and severe acute respiratory syndrome]]> https://www.researchpad.co/article/Nfca89fca-6352-45c2-a9bf-34a18298425e ]]> <![CDATA[ENTERIC CORONAVIRUS-LIKE PARTICLES IN SHEEP]]> https://www.researchpad.co/article/N63c6cff5-41d1-41fd-9d8f-924fdf34a780 ]]> <![CDATA[Spatial and temporal patterns of amphibian species richness on Tianping Mountain, Hunan Province, China]]> https://www.researchpad.co/article/N2924fcab-9b75-4945-858c-09ff83210d86

A: Species occurrence (percentage of individuals) in different sampling transects. B: Species occurrence (percentage of individuals) in different months. C: Redundancy analysis (RDA) of relationships among environmental factors and amphibian species composition. Length of environmental vector indicates degree of correlation. Only significant variables (P<0.05) are depicted. Abbreviations of environmental factors and amphibian species are listed in Supplementary Table S1 and Table S2, respectively. D: Relationship between total amphibian species richness and elevation. E: Relationship between amphibian species richness and elevation each month. F: Temporal changes in amphibian species richness over four months. Different letters on top of error bars indicate significant difference between pairwise months (P<0.05).

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<![CDATA[Preliminary study on fine structures of subcortical nuclei in rhesus monkeys by ex vivo 9.4 T MRI ]]> https://www.researchpad.co/article/N159dc537-2d1b-4ce6-bb30-20fe2e6a16e0

A: MRI images of coronal (top row), sagittal (middle row), and horizontal (bottom row) planes acquired at 3 T 3D FLASH (left column) and 9.4 T 3D FLASH (right column). Red arrows indicate location of amygdala. Amygdala subfields are more easily identified on 9.4 T MRI images than on 3 T MRI images. Region (reconstruction voxel size: 310 μm×310 μm×300 μm, yellow arrow) of claustrum is obscured in 3 T MRI slice, especially right side of brain. In contrast, 9.4 T MRI images (voxel size: 155 μm×155 μm×155 μm, red arrow) easily show boundary of claustrum. B: Representative images of 9.4 T 2D FLASH MRI, bright field (black background), and crystal violet staining of right amygdala at each age stage. One monkey brain from each age group was selected for section staining. Each row represents right-side of amygdala of sample brain, which was cut and photographed. Left column shows horizontal MRI images close to position of sections in middle column. Middle column shows sections before staining. Staining of ROIs in red frame is shown in right column. Subnuclei in amygdala can be seen clearly on MRI images and sections before and after crystal violet staining (amygdala subfield: L: Lateral nuclei, B: Basal nuclei, AB: Accessory basal nuclei). Scale bars: 1 mm. C: Manual segmentation of amygdala and hippocampus. With high-resolution, 9.4 T MRI images show clear boundaries of each amygdala subregion. 3D models show exact positions of bilateral amygdala and hippocampus in monkey brains.

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<![CDATA[Hyperactive reactive oxygen species impair function of porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43]]> https://www.researchpad.co/article/Neb2065e7-f6a4-49b1-bdaf-b9fc46715ace

A: Purified Sertoli cells were plated on dishes for culture. B: Expression levels of Sertoli cell markers, Cx43, WT1, AMH, and Sox9, were detected in purified Sertoli cells by RT-PCR, n=3. C−F: Purity of Sertoli cells was determined using IF staining against WT1 (C: Bright field; D: WT1; E: DAPI; F: Merge). G: Percentage of WT1+ cells is presented as mean percentage±SEM, n=5. H: Morphology of Sertoli cells treated with 0, 50 μmol/L H2O2, 100 μ mol/L H2O2, or 500 μmol/L H2O2 is shown. I, J: Images of DCFH-DA fluorescence were taken for positive control group (Rosup provided by the Reactive Oxygen Species Assay Kit, Beyotime) (I) and 50 μmol/L H2O2-treated group, n=5 (J). K: Apoptosis signal detection using Annexin V-FITC/PI kit. PBS- and 50 μmol/L H2O2-treated groups, n=5. L: ROS levels in Sertoli cells treated with different concentrations of H2O2 (5, 10, 20, 50, or 100 μmol/L, n=3) were analyzed by flow cytometry. M, N: Sertoli cells treated with PBS or 50 μmol/L H2O2 for 48 h were harvested to detect protein levels of WT1, ITGB1, and Cx43 using Western blotting, with data presented as means±SEM, n=3, **: P<0.01 (N). O: Schematic of Sertoli cell and SSC co-culture system. P, Q: Morphology of co-culture of SSCs maintained with PBS- (P) or H2O2- (Q) treated Sertoli cells for 48 h is displayed. R: Number of SSCs in PBS- or H2O2-treated Sertoli cells was statistically analyzed, n=3. S, T: Protein levels of DDX4, GFRA1, and PLZF in PBS- and H2O2-treated SSCs were analyzed using Western blotting; Data are presented as mean percentage±SEM, n=3, *: P<0.05, **:P<0.01 (t-test) (T). Scale bars: 20 μm.

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