ResearchPad - research-methodology-study-design https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Targeted Nutrient Modifications in Purified Diets Differentially Affect Nonalcoholic Fatty Liver Disease and Metabolic Disease Development in Rodent Models]]> https://www.researchpad.co/article/elastic_article_16171 The pathophysiology of nonalcoholic fatty liver disease (NAFLD) is still not completely understood, nor are there any approved therapies for treating this disorder, so there is a need for further research. Rodent models are typically used as surrogates for human disease and this article provides examples of how targeted nutrient manipulations in rodent diets influence NAFLD development in mice, rats, and hamsters. This article also focuses on study design details including nutritional components of the diets (and their controls), proper control diet choice, animal model/strain, and length of the study to clearly outline the strengths and drawbacks of the different dietary methods of inducing NAFLD in rodents.

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<![CDATA[Choice of Laboratory Rodent Diet May Confound Data Interpretation and Reproducibility]]> https://www.researchpad.co/article/N3f2c15c7-6922-409a-8be5-65105b36cc6d

ABSTRACT

The reproducibility of experimental data is challenged by many factors in both clinical and preclinical research. In preclinical studies, several factors may be responsible, and diet is one variable that is commonly overlooked, especially by those not trained in nutrition. In particular, grain-based diets contain complex ingredients, each of which can provide multiple nutrients, non-nutrients, and contaminants, which may vary from batch to batch. Thus, even when choosing the same grain-based diet used in the past by others, its composition will likely differ. In contrast, purified diets contain refined ingredients that offer the ability to control the composition much more closely and maintain consistency from one batch to the next, while minimizing the presence of non-nutrients and contaminants. In this article, we provide several different examples or scenarios showing how the diet choice can alter data interpretation, potentially affecting reproducibility and knowledge gained within any given field of study.

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