ResearchPad - technical-report https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Estimation of internal dose from tap water after the Fukushima Daiichi Nuclear Power Station accident using newly obtained data]]> https://www.researchpad.co/article/elastic_article_12627 Massive release of radioactive materials into the atmosphere occurred due to the Fukushima Daiichi Nuclear Power Station (FDNPS) accident in March 2011. The World Health Organization (WHO) and the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) reported the results of dose estimation to assess the health effect of the accident and both reports state that their assessments of internal and external exposure doses contain certain uncertainties due to uncertainties inherent to the basic data. Therefore, estimation of the internal dose from tap water was conducted in this study by utilizing a database of deposition calculated by an atmospheric transfer, dispersion and deposition model (ATDM) in conjunction with the newly obtained data on the volume of daily water intake obtained by a web-based survey. The median mean and 95-percentile of thyroid equivalent doses were estimated for 1-year and 10-year children and adults in 12 municipalities in the evacuation area in Fukushima prefecture. The present mean thyroid dose estimations for 1-year children (0.4–16.2 mSv) are smaller than the corresponding values in the UNSCEAR 2013 report (1.9–49 mGy). Dose-modifying factors in the Japanese or local community are discussed.

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<![CDATA[A modified scar model with controlled tension on secondary wound healing in mice]]> https://www.researchpad.co/article/elastic_article_10883 Pathological scars might cause a distorted appearance and restricted mobility, and the study of scar pathophysiology has been hindered by the absence of a reliable model. In this study, we introduce a model with a modified device to induce controlled tension on a wound healing by secondary intention to overcome the shortcomings of the model generated by Aarabi et al. We investigated and recommend an induction of 0.1 N/mm2 tension on day 7 for 14 days to mimic the characteristics of human scars. A 3.5-fold increase in scar tissue and a 2-fold increase in collagen production were induced by the modified model. Histologically, the modified method increased scar thickness. However, no significant difference was found in cell density between the two groups. This modified procedure significantly increased scar tissue, which could be used for further cellular and biomolecular research. The mechanical force applied to the wound became measurable and controllable. This method is more convenient for researchers to observe in real-time and for providing timely adjustments of the tension used in this modified model.

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<![CDATA[A technical report from the Italian SARS-CoV-2 outbreak. Postmortem sampling and autopsy investigation in cases of suspected or probable COVID-19]]> https://www.researchpad.co/article/elastic_article_9333 SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a coronavirus responsible for COVID-19 (coronavirus disease 2019) which resulted in a cluster of cases of pneumonia that originated in China around 31 December 2019 and has subsequently spread across the globe. Currently, COVID-19 represents a health emergency worldwide, leading, in severe cases, to pneumonia, severe acute respiratory syndrome, multiorgan dysfunction or failure, and death. In the context of limited scientific knowledge and evidence of SARS-CoV-2 infection, guidance is becoming increasingly necessary for pathologists who have to perform postmortem investigations on COVID-19 cases. The aim of the present report is to share a procedure applicable to cases of COVID-19-related death, particularly in cases of death without medical intervention and in the absence of an ascertained SARS-CoV-2 infection and/or COVID-19 diagnosis, therefore providing support for diagnostic activity in the present COVID-19 pandemic. For this purpose, a standard operating procedure for correct swab collection, autopsy investigation and tissue sampling is provided.

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<![CDATA[An Interdisciplinary Definition of Pornography: Results from a Global Delphi Panel]]> https://www.researchpad.co/article/N9f01c8a1-66bd-494e-b305-2f15f65ed81d

In interdisciplinary investigations into the relationships between pornography and its audiences, the issue of how to define the object of study is more complex than in studies situated within a single discipline. A Delphi panel of 38 leading pornography researchers from a wide range of disciplines was asked about various topics, including the definition of pornography. Qualitative and quantitative analyses of two rounds of survey responses suggested two different and—at first sight—incompatible definitions operating. The first was “Sexually explicit materials intended to arouse.” The second was a culturally relative definition suggesting pornography has no innate characteristics. This technical report suggests that we should encourage researchers to choose which definition they want to use in a self-reflective way depending on the needs of the project, so long as they make it explicit and justify their decision.

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<![CDATA[Modified Technique for CAD/CAM Guided Implant Planning in the Presence of Existing Hopeless Teeth]]> https://www.researchpad.co/article/N3e292fb7-47fd-4ae3-8981-fe1cb74aea8a

Immediate placement of dental implants presents many challenges, especially when partial or complete fixed restorations are the intended prosthetic outcome. With modern advancements in CAD/CAM technology, the ease and predictability of such complex cases is vastly improved. However, certain clinical situations remain that preclude the traditional implementation of this controlled approach to implant planning/placement and the current solutions to these problems each impose some level of compromise. This article describes a technique permitting both prosthetically-driven implant planning and increased surgical guide accuracy in situations where existing hopeless teeth would otherwise impede optimal treatment.

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<![CDATA[Transcript Annotation in FANTOM3: Mouse Gene Catalog Based on Physical cDNAs]]> https://www.researchpad.co/article/5989da99ab0ee8fa60ba3173

The international FANTOM consortium aims to produce a comprehensive picture of the mammalian transcriptome, based upon an extensive cDNA collection and functional annotation of full-length enriched cDNAs. The previous dataset, FANTOM2, comprised 60,770 full-length enriched cDNAs. Functional annotation revealed that this cDNA dataset contained only about half of the estimated number of mouse protein-coding genes, indicating that a number of cDNAs still remained to be collected and identified. To pursue the complete gene catalog that covers all predicted mouse genes, cloning and sequencing of full-length enriched cDNAs has been continued since FANTOM2. In FANTOM3, 42,031 newly isolated cDNAs were subjected to functional annotation, and the annotation of 4,347 FANTOM2 cDNAs was updated. To accomplish accurate functional annotation, we improved our automated annotation pipeline by introducing new coding sequence prediction programs and developed a Web-based annotation interface for simplifying the annotation procedures to reduce manual annotation errors. Automated coding sequence and function prediction was followed with manual curation and review by expert curators. A total of 102,801 full-length enriched mouse cDNAs were annotated. Out of 102,801 transcripts, 56,722 were functionally annotated as protein coding (including partial or truncated transcripts), providing to our knowledge the greatest current coverage of the mouse proteome by full-length cDNAs. The total number of distinct non-protein-coding transcripts increased to 34,030. The FANTOM3 annotation system, consisting of automated computational prediction, manual curation, and final expert curation, facilitated the comprehensive characterization of the mouse transcriptome, and could be applied to the transcriptomes of other species.

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<![CDATA[Laparoscopic Manipulation of a Probe-based Confocal Laser Endomicroscope Using a Steerable Intravascular Catheter]]> https://www.researchpad.co/article/5bbe5ee140307c7ecabd2b67

Probe-based confocal laser endomicroscopy is an emerging imaging modality that enables visualization of histologic details during endoscopy and surgery. A method of guiding the probe with millimeter accuracy is required to enable imaging in all regions of the abdomen accessed during laparoscopy. On the basis of a porcine model of laparoscopic liver resection, we report our experience of using a steerable intravascular catheter to guide a probe-based confocal laser endomicroscope.

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<![CDATA[Genotyping of OATP2 Variants in a Group of Malaysian Neonates Using High-Resolution Melting Analysis]]> https://www.researchpad.co/article/5aca4d94463d7e7f7bf36160 ]]>