ResearchPad - veterinary-science-and-veterinary-medicine https://www.researchpad.co Default RSS Feed en-us © 2020 Newgen KnowledgeWorks <![CDATA[Dataset of endometrial blood flow from pregnant and non-pregnant mares on day 7 and 8 post-ovulation]]> https://www.researchpad.co/article/Nfc52ebe6-2a1b-490d-a202-06a7372d5c55 This article provides the dataset for the use of power Doppler ultrasound to assess the equine uterus from the recent research article titled “Power Doppler can detect the presence of 7-8 days conceptuses prior to flushing in an equine embryo transfer program”(1). The vascularization of the endometrium was objectively assessed in mares by quantification of pixels in bitmap format (BMP) using computer assisted analysis of images. Fifty-two mares were examined on days 7 (26 mares) and 8 (26 mares) post-ovulation prior to performing flushing procedures for embryo recovery. Receiver operating characteristic (ROC) curves and Youden's J statistics were used to evaluate the value of the suggested variable in terms of its diagnostic value for identification of early pregnancy and to establish cut-off values allowing differentiation between pregnant and non-pregnant mares on days 7 and 8 post-ovulation.

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<![CDATA[Hygiene performance rating—An auditing scheme for evaluation of slaughter hygiene]]> https://www.researchpad.co/article/N246e13dc-d3bd-4f65-95de-d255518ef46e

The Hygiene Performance Rating scheme is developed by Animalia in Norway. This unique auditing tool for assessment of slaughter hygiene has been used in Norwegian abattoirs for the last 10 years. The Hygiene Performance Rating scheme visually evaluates and documents each operation on the slaughter line, assessing the factors that can affect the slaughter hygiene. The protocol is based on a systematic evaluation of general hygienic practices of each operation, such as the operators’ hygienic behavior and risk handling of the carcasses, along with routines and management. The scores are registered in a web-based application.

The observations are given a score from 1 to 3, where 1 means “acceptable”, 2 = “potential for improvement”, and 3 = “not acceptable”. Scores for each position is multiplied with a weight factor for hygienic impact and risk (1, 3, 6 or 12) and economic consequences (1 or 2) describing whether the necessary improvement depends on a significant investment (1) or if it is a cheap quick-fix (2) and calculated into a percentage where 100% is perfect hygiene. A presentation of results for the involved parties, including operators, is a crucial part of the implementation of the Hygiene Performance Rating scheme.

  • Systematic auditing tool for evaluating slaughter hygiene.

  • Investigate and improve slaughter techniques and routines.

  • Comprehensive approach to achieve satisfactory results for slaughter hygiene.

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<![CDATA[Optimized protocol for DNA/RNA co-extraction from adults of Dirofilaria immitis]]> https://www.researchpad.co/article/N068df7a6-7a9e-4495-b15c-2048d77eb8ab

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<![CDATA[Integrated dataset on acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin]]> https://www.researchpad.co/article/5c4f7943d5eed0c4843408a2

Data herein describe the quantitative changes in the plasma proteome in chickens challenged with lipopolysaccharide (LPS), a bacterial endotoxin known to stimulate the host innate immune system obtained by shotgun quantitative proteomic tandem mass tags approach using high-resolution Orbitrap technology. Statistical and bioinformatic analyses were performed to specify the effect of bacterial endotoxin. Plasma from chicken (N=6) challenged with Escherichia coli (LPS) (2 mg/kg body weight) was collected pre (0 h) and at 12, 24, 48, and 72 h post injection along with plasma from a control group (N=6) challenged with sterile saline. Protein identification and relative quantification were performed using Proteome Discoverer, and data were analysed using R. Gene Ontology terms were analysed by the Cytoscape application ClueGO based on Gallus gallus GO Biological Process database, and refined by REVIGO. Absolute quantification of several acute phase proteins, e.g. alpha-1-acid glycoprotein (AGP), serum amyloid A (SAA) and ovotrensferrin (OVT) was performed by immunoassays to validate the LC-MS results. The data contained within this article are directly related to our research article”Quantitative proteomics using tandem mass tags in relation to the acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin” [1]. The raw mass spectrometric data generated in this study were deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009399 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD009399).

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